Renilla luciferase (RLuc) is a new type of luciferase with a size of 36 kD. The enzyme can catalyze enterocelins that can penetrate cell membranes under oxygen and emit fluorescence (maximum wavelength 480 nm), the reaction is so efficient that almost all of the energy input to the reaction is converted into light. LZCapAG(3'Acm) RLuc mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm) cap analogs. The Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression compared to the Cap0 structure and other commercially available Cap1 structures. LZCapAG(3'Acm) RLuc mRNA has a poly A and an optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation for better expression. LZCapAG(3'Acm) RLuc mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.
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