Red fluorescent protein (RFP) is a protein isolated from the Pacific Ocean sea anemone associated Discosomasp that can emit red fluorescence under ultraviolet irradiation at a wavelength of 587 nm. LZCapAG(3'Acm) RFP mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm). Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) RFP mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve better expression effect. The immunogenicity of mRNA can be effectively reduced after m1Ψ modification. LZCapAG(3'Acm) RFP mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.
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