LZCapAG(3'Acm) mCherry mRNA

LZCapAG(3'Acm) mCherry mRNA

The excitation/emission wavelengths of mCherry red fluorescent protein were 587 nm/610 nm, respectively. LZCapAG(3'Acm) mCherry mRNA was synthesized and purified by one-step transcription of T7 transcriptase and LZCapAG(3'Acm) cap analogs from a linear template. After co-transcription, LZCapAG(3'Acm) generates mRNA with Cap1 structure. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) has a more efficient and durable expression. LZCapAG(3'Acm) mCherry mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve better expression effect. The immunogenicity of mRNA can be effectively reduced after m1Ψ modification. LZCapAG(3'Acm) mCherry mRNA will express red fluorescent protein after entering cells, which is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

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