Firefly Luciferase (Firefly luc) expresses a luciferase protein originally extracted from fireflies. The enzyme relies on ATP to catalyze the oxidation of D-fluorescein to produce chemiluminescence at a wavelength of about 560 nm. Firefly luc is a commonly used bioluminescence reporter gene used in gene regulation and function studies. It emits biofluorescence in the presence of the substrate fluorescein. Firefly luc mRNA is synthesized and purified by one-step transcription from a linear template using T7 transcriptase and LZCapAG(3'Acm) cap analogs. The mRNA will express luciferase protein when it enters the cell. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) Firefly luc mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.
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