Enhanced Green Fluorescent Protein (eGFP) is an enhanced variant of wild-type GFP with a maximum excitation/emission wavelength of 488 nm/509 nm, respectively. eGFP, as a common reporter molecule, emits a strong green fluorescence when excited and can be detected by fluorescence microscopy or flow cytometry. eGFP mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG cap analogs. The Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression compared to the Cap0 structure and other commercially available Cap1 structures. When this mRNA enters the cell, it will express a green fluorescent protein. eGFP mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.
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