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Under the guidance of sgRNA, Cas9 protein can introduce DNA double-strand breaks (DSBS) at designated genomic locations, and cells will then activate endogenous DNA repair programs, namely non-homologous end junction (NHEJ) or homologous recombination repair mechanism (HDR), to repair the target DSB, and realize the recognition and cutting of genomic DNA targets. LZCapAG(3'Acm) Cas9 mRNA can be used with purified sgRNA to perform the cutting function with sgRNA through the expressed Cas9 protein for CRISPR/Cas genome editing. LZCapAG(3'Acm) Cas9 mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm) cap analogs. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) Cas9 mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve a better expression effect. LZCapAG(3'Acm) Cas9 mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and gene editing experiments.
Chemical Structure:
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