CALF Red 610 is a novel oxanthracene fluorescent dye designed for qPCR instruments. The attachment chemistry of CALF Red dyes to biomolecules eliminates the problem of multiple isomers, which results in dye labels that are easier to prepare. CALF dyes can be used in oligonucleotide synthesis in the form of CPG and phosphoramides, allowing for more efficient labeling and fewer purification steps than post-synthetic labeling. CALF dyes are sensitive to light, and prolonged exposure to light can cause photobleaching. CALF Red 610 has an excitation wavelength of 590 nm, an emission wavelength of 610 nm, a single RP-HPLC peak and a well-defined emission spectrum, and can be paired with BHQ-1 or BHQ-2 to achieve efficient quenching of various probe forms. The CAlF Red 610 is a replacement for Texas Red and ROX.
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