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5-Methylcytidine-5'-triphosphate, sodium salt is a modified nucleotide used in molecular biology. It contains a methyl group attached to the 5th carbon of cytidine's pyrimidine ring and three phosphate groups esterified to the ribose sugar's 5' position. This compound, in its sodium salt form, is soluble in water. It's commonly used as a substrate in enzymatic reactions, particularly in processes like transcription and reverse transcription, where it can introduce methylation marks into RNA molecules. These modifications influence RNA structure and function, playing vital roles in gene expression regulation and cellular processes. It's a valuable tool for studying RNA modifications and epigenetic regulation.
Chemical Structure:
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