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5'-Biotin CE Phosphoramidite
5'-Biotin Phosphoramidite for direct labeling of synthetic oligonucleotides has the following characteristics:
- Soluble in acetonitrile at concentrations useful for DNA synthesis.
- Include the DMT group used for cassette purification, which is essential for the preparation of biotinylated PCR primers because cross-contamination may occur during HPLC purification.
- To develop diagnostic probes, biotin phosphoramidite can be branched to allow the introduction of multiple biotins at the 3'or 5'end.
* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.
Catalog
BRP-02218
Synonyms
DMT-Biotin-C6 Phosphoramidite; Phosphoramidous acid, N,N-bis(1-methylethyl)-, 6-[[5-[(3aS,4S,6aR)-1-[bis(4-methoxyphenyl)phenylmethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]hexyl 2-cyanoethyl ester; Phosphoramidous acid, bis(1-methylethyl)-, 6-[[5-[1-[bis(4-methoxyphenyl)phenylmethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]hexyl 2-cyanoethyl ester, [3aS-(3aα,4β,6aα)]-; 5'-Biotin Phosphoramidite; N1-(4,4'-Dimethoxytrityl)-biotinyl-6-aminohexyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
CAS
135137-87-0
IUPAC Name
5-[(3aR,6S,6aS)-3-[bis(4-methoxyphenyl)-phenylmethyl]-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]-N-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexyl]pentanamide
Molecular Weight
846.08
Molecular Formula
C46H64N5O6PS
Canonical SMILES
CC(C)N(C(C)C)P(OCCCCCCNC(=O)CCCCC1C2C(CS1)N(C(=O)N2)C(C3=CC=CC=C3)(C4=CC=C(C=C4)OC)C5=CC=C(C=C5)OC)OCCC#N
InChI
InChI=1S/C46H64N5O6PS/c1-34(2)51(35(3)4)58(57-32-16-29-47)56-31-15-8-7-14-30-48-43(52)20-13-12-19-42-44-41(33-59-42)50(45(53)49-44)46(36-17-10-9-11-18-36,37-21-25-39(54-5)26-22-37)38-23-27-40(55-6)28-24-38/h9-11,17-18,21-28,34-35,41-42,44H,7-8,12-16,19-20,30-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,58?/m0/s1
InChIKey
RBXYKQBFPLXSJS-RYQQVEDISA-N
Boiling Point
934.9±65.0 °C at 760 mmHg
Purity
>95% by HPLC
Appearance
Off-white Solid
Storage
Store at -20 °C
Symbol
DMT-Biotin-C6 Amidite
Formulation
Dilute with anhydrous acetonitrile
Chemical Structure:
